3

1/19/11

(A;Class Meetings)

1/21/11

(C)

No Classes!

.

1/25/11

(E)

Lesson Unit/Topic

• Introduce Course Syllabus.

• Discuss Lab Safety.

Read and sign the lab safety contract and bring it with you next class.

Also finish the measurement practice problems that you started in class.

Have a good week!

1/27/11

(A)

Lesson Unit/Topic

• SNOW DAY!

.

1/31/11

(C)

Lesson Unit/Topic

• Review Conversions & measurements.

• 'Micropipetting activity'.

 Finish the problems on the worksheet that you received in class. Try the bottom of the page as well --> it is referring to the display window in the micropipette.

Have a good week!

2/2/11

(E; Advisee Mtg during Break + 15)

Lesson Unit/Topic

• Introduce Electrophoresis.

• Review Measurement problems.

Here's your Electrophoresis assignment.

Bring this with you next class.

Have a good day!

2/4/11

(A; First Friday)

Lesson Unit/Topic

• Discuss Electrophoresis.

• Review Measurements.

2/8/11

(C)

Lesson Unit/Topic

• QUIZ.

• Electrophoresis Learning Cycle.

 Your Electrophoresis lab with all questions answered is due  next class.

You must use a pencil to write in the worksheet.

Have a good week!

2/10/11

(E)

Lesson Unit/Topic

• Review Electrophoresis.

• Review Measurements.

• DNAi scavenger hunt.

Study for your TEST next class on Electrophoresis, Measurements (Conversions) & DNAi scavenger hunt. Just so you all know, calculators will not be allowed on the TEST.

Have a good weekend!

2/14/11

(A)

Lesson Unit/Topic

• TEST .

• Start 'Romanov webquest'.

Review Pedigrees from this tutorial and take notes in your binder.

Have a good week!

2/16/11

(C; Class Meetings)

Lesson Unit/Topic

• Introduce Pedigrees.

• Romanov webquest.

Practice pedigree worksheet- analyze the pedigrees on page 27 --> for all of these 10 pedigrees, identify the inheritance pattern and in your binder write out 1-2 sentences on how and why you reached your conclusion

Have a good week!.

2/18/11

(E; Mid-Quarter Reports)

Lesson Unit/Topic

• Pedigree practice.

• Finish Romanov mystery webquest.

From your binder analyze pedigrees on page 28. Number these pedigrees 1-10 (left to right) and for each identify the inheritance pattern and in your binder write out 1-2 sentences on how and why you reached your conclusion.

Have a relaxing Break!

WINTER BREAK!

3/1/11

(A)

Lesson Unit/Topic

 Review class-notes.

Study for your Quiz next class on pedigrees + Romanov mystery webquest.

Pedigree on page 29: Pedigree #2  is X-linked recessive, #3 is Autosomal recessive, and #4 is X-linked dominant.

 Have a good week!

3/3/11

(C)

Lesson Unit/Topic

• Quiz on Pedigrees + Romanov webquest.

• Introduce DNA structure.

Review class-notes and finish the DNA base pair sheet (page 24) that you started in class. Also finish the DNA structure-function worksheet (pages 52-53) in your binder.

Have a good weekend!

3/7/11

(E)

Lesson Unit/Topic

• Discuss DNA Replication.

• 'DNA- protein' webquest.

Study for your Quiz next class on Central dogma, DNA structure and DNA Replication.

Also one of the two pages of the webquest should be finished before next class.

Have a good week!

3/9/11

(A; Ash Wednesday Liturgy)

Lesson Unit/Topic

• QUIZ

• Discuss Transcription/RNA editing.

 If you missed the Quiz, you will be taking the Quiz next class. Thanks!

Finish the second page of the webquest. Your answers should be neatly written or typed in complete sentences and ready to be handed in. If you have typed them, you should have the printout ready before you come to class.

Have a good week!

3/11/11

(C)

Lesson Unit/Topic

• Describe Translation.

• Review

• Class works on the family tree.

• Bring the DNA webquest with you next class.
• Finish the 'Breaking the Code' worksheet handed out in class.
• Research the clinical symptoms and come prepared to present next class.
• Draw the family pedigree in word, print it and bring it with you next class.

Have a good weekend!

3/15/11

(E; Spirit Week)

Lesson Unit/Topic

• Review.

• Clinical symptoms presentations.

Study for your test next class on Central Dogma, DNA structure, Replication, Transcription, Translation, RNA editing, pedigrees + relevant worksheets.

(Romanov webquest will not be on the test).

Have a good week!

3/17/11

(A; Spirit Week)

Lesson Unit/Topic

• TEST.

RELAX! REST!

3/21/11

(C)

Lesson Unit/Topic

• Introduce 'PCR'.

• Class sorts through family symptoms.

 Use your clinical symptoms and OMIM to come up with a putative diagnosis for your family. You should have a genetic disorder, its gene locus and your family pedigree with affected individuals and carriers (if any) marked on the pedigree. This typed pedigree alongwith a page that shows your research should be handed in next class.

Your PCR flowchart (typed) is also due next class.

Have a good week!

3/23/11

(E)

Lesson Unit/Topic

• Class sets up 'PCR'.

• 'PCR' tutorial.

Finish page 69 in your binder. Also design primers (5 base long) for the following two DNA templates:

1.   5' GAT TGC CGA GGA ACT TAC GCT CTA A 3'

2.   5' ACG GTA ATT TTC GCA AAC CTT CGG GCA AC 3'

Have a good week!

3/25/11

(A; Grades Close)

Lesson Unit/Topic

• Analyze PCR results via electrophoresis.

• Discuss lab report.

• 'Which little piggy' case study.

The first part of your Vee map lab report (Title, Focus Question, Concept Map, Variables, Hypothesis, Flowchart, Knowledge Claim/Value Claim) is due next class (typed and printed -- you will not be allowed to leave class to print the assignment - sorry!). It will not be possible for me to read it off your computer screen either. Thanks!

Here's the Vee map template.

Read pages 5-7 of the PCR handout to get the information for your Knowledge claim/Value claim.

Have a good weekend!

3/29/11

(C)

Lesson Unit/Topic

• 'PCR analysis' --> pages 5-7 of the PCR handout

• mtDNA sequencing -- Introduction.

 Your PCR worksheet and the Article summary are due next class.

Have a good day!

4/1/11

(E; First Friday)

Lesson Unit/Topic

• 'PCR lab write-up'.

• Case study.

Finish the Transformation and Conclusion for your PCR lab (regular page no Vee map)--> due next class.

Also from pages 78-79 in your binder, make the flowchart for the mtDNA PCR experiment.

 Have a good weekend!

4/5/11

(A)

Lesson Unit/Topic

• 'Mt DNA PCR lab'.

 Read pages 74  to 76 in your binder and complete this worksheet -- please print it and bring it with you.

Have a good day!

4/7/11

(C)

Lesson Unit/Topic

• Set up mtDNA  PCR.

• Class finishes 'Which Little Piggy' Case study?

Review class-notes on PCR and design 5 base long primers for the following DNA templates:

• 5' ATG GCC CTG GGG TGC CTA ATT GAT TGC CGA 3'

• 5' ATG GCA ATT CTA GTC ACT TAA AGG3'

Also in your binders answer the following questions:

• How many copies of a gene would you have after doing PCR for 4 cycles and 8 cycles?

• Which step of PCR would be affected if you added the wrong template?

• Which step of PCR would be affected if you did not add the dNTPs by mistake?

Have a good weekend!

4/11/11

(E; 1:10; Stuco Speeches and Elections)

Lesson Unit/Topic

• Class sets up mtDNA PCR.

• Review for Quiz.

Study for your Quiz on PCR next class. The quiz includes PCR --notes +lab + pages 74-77 +mtDNA PCR notes +lab.

Start your PCR lab today since it will be due on 4/15/11.

Have a good day!

4/13/11

(A)

Lesson Unit/Topic

• 'PCR Quiz'.

• mtDNA PCR gel.

Finish the first page of your mtDNA PCR lab report-- Focus questions, Concept Map, Variables, Hypothesis, Flowchart, Knowledge Claim and Value Claim -- bring the printout with you.

Have a good day!

4/15/11

(C; Junior Picnic)

Lesson Unit/Topic

• Discuss how restriction enzymes work in gene cloning.

• mtDNA PCR instructions.

• Gel picture.

Finish the Transformation & Conclusion part of your lab report and bring in the entire lab report on 4/27/11.

Have a nice break!

SPRING AND EASTER BREAK!

4/27/11

(E; 1:10; Class  Elections)

Lesson Unit/Topic

• Introduce Restriction Digest.

• Review for test.

Study for your TEST next class on PCR

 and the Which little Piggy - case study?'.

4/29/11

(A; Advisee Mtg; Senior Mid-Quarter Reports)

Lesson Unit/Topic

• 'PCR TEST'.

Have a good weekend!

5/3/11

(C)

Lesson Unit/Topic

• 'Restriction digests.

Finish any part of the in-class assignments that you have remaining and bring them with you next class.

Have a good week!

5/5/11

(E)

Lesson Unit/Topic

• Discuss Restriction Analysis for circular plasmid.

 Remember to get your homework worksheet  (May 5th homework) from the hand in folder.

Have a good weekend!

5/9/11

(A)

Lesson Unit/Topic

• Class sets up restriction digest.

• Discuss Restriction enzyme lab write-up.

5/11/11

(C)

Lesson Unit/Topic

• Discuss 'Druid Dracula' case study.

• Review.

Come prepared for your Quiz next class on restriction analysis.

5/13/11

(E)

Lesson Unit/Topic

• QUIZ on restriction analysis.

• Run restriction digests on gel.

Answer questions 1-5 on the sequencing worksheet.

Have a good weekend!

5/17/11

(A)

Lesson Unit/Topic

• Introduce Sequencing.

• 'Beetle STR crime scene' analysis.

Have a good week!

5/19/11

(C; 1:10 May Crowning)

Lesson Unit/Topic

• Continue with DNA sequencing.

Finish DNA Sequencing worksheet- pages 106, 108-109.

Your Restriction Digest lab is due next on Monday 5/23/11 by 8AM in homeroom 345 (No  exceptions please). Any lab report received after that time will be marked late.

Have a good weekend!

5/24/11

(E)

Lesson Unit/Topic

• Cycle sequencing.

• 'Cycle Sequencing' activity.

Study for your Test next class on Restriction Enzymes, STR analysis and Sequencing (Traditional & Cycle -- parts that have been covered in class).

Please bring in the homework that was due today (pages 106,108-109) with you next class (5/26/11).

 Have a good week!

5/26/11

(A; Senior Report Cards On-Line)

Lesson Unit/Topic

• TEST on Restriction Analysis, STRs and sequencing.

.

Organize your binder as directed in preparation for Finals. Also for each of the units covered type up 4 questions that will eventually become part of a class-wide Review packet. Print them out and bring the print-out with you next class.

Have a good week!

5/31/11

(C)

Lesson Unit/Topic

• Parts of the sequencer.

• Pedigree practice.

• Review.

Go over the Review packet -- come fully prepared with questions that your Group worked on and also bring questions that you would like the other groups to clarify for you.

Have a good week!

6/2/11

(E; Ascension Thursday)

Lesson Unit/Topic

Prepare for Final Exam